作者
Rebecca Gum, Ernst Lengyel, Jose Juarez, Ji Hshiung Chen, Hiroshi Sato, Motoharu Seiki, Douglas Boyd
发表日期
1996/5/3
期刊
Journal of Biological Chemistry
卷号
271
期号
18
页码范围
10672-10680
出版商
Elsevier
简介
The 92-kDa type IV collagenase (92-kDa gelatinase B also referred to as MMP-9), which plays a critical role in extracellular matrix degradation, is regulated by growth factors that mediate their effects through the ras proto-oncogene. The current study was undertaken to determine the transcriptional requirements for the induction of 92-kDa gelatinase B expression by an activated ras oncogene. Transfection of OVCAR-3 cells with an expression vector encoding an activated Ha-ras increased 92-kDa gelatinolytic activity and stimulated (over 10-fold) the activity of a CAT reporter driven by 670 nucleotides of 5‘ flanking sequence of the 92-kDa gelatinase B gene. Transient assays using a CAT reporter driven by 5‘ deleted fragments of the 92-kDa gelatinase B promoter indicated that a region spanning −634 to −531 was required for optimal induction of the promoter. The individual deletion, or mutation, of a PEA3/ets(−540 …
引用总数
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R Gum, E Lengyel, J Juarez, JH Chen, H Sato, M Seiki… - Journal of Biological Chemistry, 1996