作者
Eileen Pedraza, Maria M Coronel, Christopher A Fraker, Camillo Ricordi, Cherie L Stabler
发表日期
2012/3/13
期刊
Proceedings of the National Academy of Sciences
卷号
109
期号
11
页码范围
4245-4250
出版商
National Academy of Sciences
简介
A major hindrance in engineering tissues containing highly metabolically active cells is the insufficient oxygenation of these implants, which results in dying or dysfunctional cells in portions of the graft. The development of methods to increase oxygen availability within tissue-engineered implants, particularly during the early engraftment period, would serve to allay hypoxia-induced cell death. Herein, we designed and developed a hydrolytically activated oxygen-generating biomaterial in the form of polydimethylsiloxane (PDMS)-encapsulated solid calcium peroxide, PDMS-CaO2. Encapsulation of solid peroxide within hydrophobic PDMS resulted in sustained oxygen generation, whereby a single disk generated oxygen for more than 6 wk at an average rate of 0.026 mM per day. The ability of this oxygen-generating material to support cell survival was evaluated using a β cell line and pancreatic rat islets. The …
学术搜索中的文章
E Pedraza, MM Coronel, CA Fraker, C Ricordi… - Proceedings of the National Academy of Sciences, 2012