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To assess porcine urothelial cell cultures and the in vitro induction of urothelial stratification in long‐term cultures, to study their morphological, functional and genetic behaviour, and thus provide potential autologous urothelium for tissue‐engineered substitutes for demucosalized gastric or colonic tissue.

Primary cultures of porcine urothelium were established and the cells passaged thereafter. Cell specificity was confirmed by cytokeratin analysis, cell membrane stability assessed using lactate dehydrogenase leakage, cell de‐differentiation by γ‐glutamyl transferase activity and genomic stability by karyotype investigations. Histology and scanning electron microscopy were performed to study the cultured cells and the stratified constructs. Furthermore, collagen matrices were tested as cell scaffolds.

The cells were cultured for 180 days; 10 subcultures were established …