作者
Tim Ebert, Sharon Smith, Greg Pancari, Desmond Clark, Richard Hampton, Susan Secore, Victoria Towne, Hongxia Fan, Xin-Min Wang, Xiaoqing Wu, Robin Ernst, Barrett R Harvey, Adam C Finnefrock, Fubao Wang, Charles Tan, Eberhard Durr, Leslie Cope, Annaliesa Anderson, Zhiqiang An, Tessie McNeely
发表日期
2010/1/1
期刊
Human antibodies
卷号
19
期号
4
页码范围
113-128
出版商
IOS Press
简介
A fully human monoclonal antibody (CS-D7, IgG 1) specific for the iron regulated surface determinant B (IsdB) of Staphylococcus aureus was isolated from the Cambridge Antibody Technology (CAT) scFv antibody library. As compared to previously described IsdB specific murine monoclonals, CS-D7 has a unique, non-overlapping binding site on IsdB, and exhibits increased in vivo activity. The antibody recognizes a conformational epitope spanning amino acids 50 to 285 and has a binding affinity of 340 (±75) pM for IsdB. CS-D7 bound to a wide variety of S. aureus strains, but not to an isdB deletion mutant. The antibody mediated opsonophagocytic (OP) killing in vitro and mediated significant protection in vivo. In a murine lethal sepsis model, the antibody conferred protection from death when dosed prior to challenge, but not when dosed after challenge. Importantly, in a central venous catheter (CVC) model in rats …
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T Ebert, S Smith, G Pancari, D Clark, R Hampton… - Human antibodies, 2010