作者
Nicole L Sullivan, Morgan A Reuter-Monslow, Janet Sei, Eberhard Durr, Carl W Davis, Cathy Chang, Megan McCausland, Andreas Wieland, David Krah, Nadine Rouphael, Aneesh K Mehta, Mark J Mulligan, Bali Pulendran, Rafi Ahmed, Kalpit A Vora
发表日期
2018/7/15
期刊
Journal of virology
卷号
92
期号
14
页码范围
10.1128/jvi. 00269-18
出版商
American Society for Microbiology
简介
Herpes zoster (HZ) (shingles) is the clinical manifestation of varicella-zoster virus (VZV) reactivation. HZ typically develops as people age, due to decreased cell-mediated immunity. However, the importance of antibodies for immunity against HZ prevention remains to be understood. The goal of this study was to examine the breadth and functionality of VZV-specific antibodies after vaccination with a live attenuated HZ vaccine (Zostavax). Direct enumeration of VZV-specific antibody-secreting cells (ASCs) via enzyme-linked immunosorbent spot assay (ELISPOT assay) showed that Zostavax can induce both IgG and IgA ASCs 7 days after vaccination but not IgM ASCs. The VZV-specific ASCs range from 33 to 55% of the total IgG ASCs. Twenty-five human VZV-specific monoclonal antibodies (MAbs) were cloned and characterized from single-cell-sorted ASCs of five subjects (>60 years old) who received Zostavax …
学术搜索中的文章
NL Sullivan, MA Reuter-Monslow, J Sei, E Durr… - Journal of virology, 2018