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In this first systematic study, we describe the influence of various surfactants and polymers on aggregation stability and antibacterial activity of silver nanoparticles (NPs) prepared by a modified Tollens process. The surfactant/polymer stabilizing effect was monitored using the newly established method based on a titration of the aqueous dispersion of the silver NPs by aqueous solution of poly(diallyldimethylammonium) chloride (PDDA). The aggregation process was evaluated by the dynamic light scattering (DLS) and UV/vis spectra measurements and finally confirmed by TEM. Among all of the investigated modifiers, two surfactants (sodium dodecyl sulfate-SDS and polyoxyethylenesorbitane monooleate-Tween 80) and one polymer (polyvinylpyrrolidone-PVP 360) exhibit superior stabilization of the silver NP dispersions against the process of aggregation. The differences in the stabilization ability of various tested …