作者
Malte Tiburcy, James E Hudson, Paul Balfanz, Susanne Schlick, Tim Meyer, Mei-Ling Chang Liao, Elif Levent, Farah Raad, Sebastian Zeidler, Edgar Wingender, Johannes Riegler, Mouer Wang, Joseph D Gold, Izhak Kehat, Erich Wettwer, Ursula Ravens, Pieterjan Dierickx, Linda W Van Laake, Marie Jose Goumans, Sara Khadjeh, Karl Toischer, Gerd Hasenfuss, Larry A Couture, Andreas Unger, Wolfgang A Linke, Toshiyuki Araki, Benjamin Neel, Gordon Keller, Lior Gepstein, Joseph C Wu, Wolfram-Hubertus Zimmermann
发表日期
2017/5/9
期刊
Circulation
卷号
135
期号
19
页码范围
1832-1847
出版商
Lippincott Williams & Wilkins
简介
Background
Advancing structural and functional maturation of stem cell–derived cardiomyocytes remains a key challenge for applications in disease modeling, drug screening, and heart repair. Here, we sought to advance cardiomyocyte maturation in engineered human myocardium (EHM) toward an adult phenotype under defined conditions.
Methods
We systematically investigated cell composition, matrix, and media conditions to generate EHM from embryonic and induced pluripotent stem cell–derived cardiomyocytes and fibroblasts with organotypic functionality under serum-free conditions. We used morphological, functional, and transcriptome analyses to benchmark maturation of EHM.
Results
EHM demonstrated important structural and functional properties of postnatal myocardium, including: (1) rod-shaped cardiomyocytes with M bands assembled as a functional syncytium; (2) systolic twitch forces at a …
学术搜索中的文章
M Tiburcy, JE Hudson, P Balfanz, S Schlick, T Meyer… - Circulation, 2017