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Objective

This study aims to evaluate the reproducibility, sensitivity and specificity of hyperspectral imaging of individual spermatozoa immobilized prior to intracytoplasmic sperm injection (ICSI) to establish a unique fingerprint that unequivocally identifies each cell.

Materials and Methods

This was a pilot study performed in IVIRMA Valencia. The HinaLea 4200M Microscope System hyperspectral camera was coupled to an Olympus IX73 inverted microscope. TruScope 1.1. 17 was used to obtain the hypercubes. MATLAB 2016a was used to select the most informative wavelengths that allowed for the definition of the borders of each spermatozoon. A mask was generated in python and applied to each hypercube in MATLAB, to retrieve only the spectra for that specific sperm cell avoiding interference from other cells or debris in the image. The masks were focused on the head and mid-section of the spermatozoa. To …