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A Cr(VI) resistant bacterial strain SDCr-5, identified as Ochrobactrum intermedium on the basis of 16S rRNA gene sequencing, was tolerant to high concentrations of Cr(VI) up to 15mgml⁻¹ in acetate minimal medium. O. intermedium SDCr-5 reduced Cr(VI) under a wide range of concentrations from 100 to 1500μgml⁻¹ and reduction was optimum at 37°C and pH 7. It reduced 200 and 721μgml⁻¹ Cr(VI) within 72 and 96h, respectively. The rate of Cr(VI) reduction increased with concentration from 100 to 1500μg ml⁻¹. The presence of heavy metal cations such as Cu²⁺, Co²⁺, Mn²⁺ and Ni²⁺ stimulated Cr(VI) reduction. Strain SDCr-5 might be useful for Cr(VI) detoxification under a wide range of environmental conditions.