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In tropical and sub-tropical regions, biomass carbon (C) losses through forest degradation are recognized as central to global terrestrial carbon cycles. Accurate estimation of forest biomass C is needed to provide information on C fluxes and balances in such systems. The objective of this study was to develop generalized biomass models using harvest data covering tropical semi-evergreen, tropical wet evergreen, sub-tropical broad leaved, and sub-tropical pine forest in North East India (NEI). Among the four biomass estimation models (BEMs) tested AGB_est = 0.32(D²Hδ)^0.75 × 1.34 and AGB_est = 0.18D^2.16 × 1.32 were found to be the first and second best models for the different forest types in NEI. The study also revealed that four commonly used generic models developed by Chambers (2001), Brown (1989), Chave (2005) and Chave (2014) overestimated biomass stocks by 300–591 kg tree⁻¹, while our highest rated model overestimated biomass by 197 kg tree⁻¹. We believe the BEMs we developed will be useful for practitioners involved in remote sensing, biomass estimation and in projects on climate change mitigation, and payment for ecosystem services. We recommend future studies to address country scale estimation of forest biomass covering different forest types.