作者
João Frade, Shoma Nakagawa, Paola Cortes, Umberto Di Vicino, Neus Romo, Frederic Lluis, Maria Pia Cosma
发表日期
2019/10/16
期刊
Science Advances
卷号
5
期号
10
页码范围
eaax4199
出版商
American Association for the Advancement of Science
简介
Cells with high ploidy content are common in mammalian extraembryonic and adult tissues. Cell-to-cell fusion generates polyploid cells during mammalian development and tissue regeneration. However, whether increased ploidy can be occasionally tolerated in embryonic lineages still remains largely unknown. Here, we show that pluripotent, fusion-derived tetraploid cells, when injected in a recipient mouse blastocyst, can generate diploid cells upon ploidy reduction. The generated diploid cells form part of the adult tissues in mouse chimeras. Parental chromosomes in pluripotent tetraploid cells are segregated through tripolar mitosis both randomly and nonrandomly and without aneuploidy. Tetraploid-derived diploid cells show a differentiated phenotype. Overall, we discovered an unexpected process of controlled genome reduction in pluripotent tetraploid cells. This mechanism can ultimately generate diploid …
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J Frade, S Nakagawa, P Cortes, U Di Vicino, N Romo… - Science Advances, 2019