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To analyze and compare the total proteome of aqueous humor (AH) from patients having primary angle closure glaucoma (PACG), primary open angle glaucoma (POAG) and age-related cataract.

Aqueous humor was collected from age-matched PACG, POAG and cataract patients who underwent surgery, and it was immediately stored at − 80 °C until analysis. From each sample, 25 µg of total protein was subjected to trypsin digestion and subsequently LC–MS/MS analysis was performed for the deep proteome analysis. The data acquired after the LC–MS/MS analysis were analyzed using Proteome Discoverer 1.4. The identified peptide matches were validated using percolator, at less than 1% false discovery rates.

A total of 625, 594 and 636 proteins were identified in PACG …