作者
Swati Shukla, Arpna Srivastava, Sunil Kumar, Usha Singh, Sandeep Goswami, Bhavna Chawla, Mandeep Singh Bajaj, Seema Kashyap, Jasbir Kaur
发表日期
2017
期刊
International journal of ophthalmology
卷号
10
期号
11
页码范围
1655
出版商
Press of International Journal of Ophthalmology
简介
AIM
To elucidate the mechanism of multidrug resistance in retinoblastoma, and to acquire more insights into in vivo drug resistance.
METHODS
Three anticancer drug resistant Y79 human RB cells were generated against vincristine, etoposide or carboplatin, which are used for conventional chemotherapy in RB. Primary cultures from enucleated eyes after chemotherapy (PCNC) were also prepared. Their chemosensitivity to chemotherapeutic agents (vincristine, etoposide and carboplatin) were measured using MTT assay. Western blot analysis was performed to evaluate the expression of p53, Bcl-2 and various multidrug resistant proteins in retinoblastoma cells.
RESULTS
Following exposure to chemotherapeutic drugs, PCNC showed less sensitivity to drugs. No significant changes observed in the p53 expression, whereas Bcl-2 expression was found to be increased in the drug resistant cells as well as in PCNC …
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S Shukla, A Srivastava, S Kumar, U Singh, S Goswami… - International journal of ophthalmology, 2017
