作者
A Brunetti, M Tavazza, E Noris, R Tavazza, P Caciagli, G Ancora, S Crespi, GP Accotto
发表日期
1997/7
期刊
Molecular Plant-Microbe Interactions
卷号
10
期号
5
页码范围
571-579
出版商
The American Phytopathological Society
简介
A truncated version of the C1 gene of tomato yellow leaf curl geminivirus (TYLCV), encoding the first 210 amino acids of the multifunctional Rep protein, was introduced by Agrobacterium transformation into Lycopersicon esculentum cv. Moneymaker plants under the transcriptional control of an enhanced cauliflower mosaic virus 35S promoter. One R0 plant (line 47) carrying the C1 gene in two loci (A and B) and accumulating the truncated Rep protein (T-Rep), was crossed with either a wild-type plant, or a C1 antisense plant (line 10). The wild type (wt) × 47 progeny were phenotypically homogeneous, contained either A or B locus, expressed high levels of T-Rep protein, had a “curled” phenotype, and were resistant to TYLCV when challenged either by agroinfection or by the vector Bemisia tabaci. In the 10 × 47 progeny, plants carrying only the sense gene behaved like the wt × 47 progeny, while those containing …
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A Brunetti, M Tavazza, E Noris, R Tavazza, P Caciagli… - Molecular Plant-Microbe Interactions, 1997