作者
Collin D Heer, Daniel J Sanderson, Lynden S Voth, Yousef MO Alhammad, Mark S Schmidt, Samuel AJ Trammell, Stanley Perlman, Michael S Cohen, Anthony R Fehr, Charles Brenner
发表日期
2020/12/25
期刊
Journal of Biological Chemistry
卷号
295
期号
52
页码范围
17986-17996
出版商
Elsevier
简介
Poly(ADP-ribose) polymerase (PARP) superfamily members covalently link either a single ADP-ribose (ADPR) or a chain of ADPR units to proteins using NAD as the source of ADPR. Although the well-known poly(ADP-ribosylating) (PARylating) PARPs primarily function in the DNA damage response, many noncanonical mono(ADP-ribosylating) (MARylating) PARPs are associated with cellular antiviral responses. We recently demonstrated robust up-regulation of several PARPs following infection with murine hepatitis virus (MHV), a model coronavirus. Here we show that SARS-CoV-2 infection strikingly up-regulates MARylating PARPs and induces the expression of genes encoding enzymes for salvage NAD synthesis from nicotinamide (NAM) and nicotinamide riboside (NR), while down-regulating other NAD biosynthetic pathways. We show that overexpression of PARP10 is sufficient to depress cellular NAD …
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CD Heer, DJ Sanderson, LS Voth, YMO Alhammad… - Journal of Biological Chemistry, 2020