作者
Marie Winther-Sørensen, Katrine D Galsgaard, Alberto Santos, Samuel AJ Trammell, Karolina Sulek, Rune E Kuhre, Jens Pedersen, Daniel B Andersen, Anna S Hassing, Morten Dall, Jonas T Treebak, Matthew P Gillum, Signe S Torekov, Johanne A Windeløv, Jenna E Hunt, Sasha AS Kjeldsen, Sara L Jepsen, Catherine G Vasilopoulou, Filip K Knop, Cathrine Ørskov, Mikkel P Werge, Hanne Cathrine Bisgaard, Peter Lykke Eriksen, Hendrik Vilstrup, Lise Lotte Gluud, Jens J Holst, Nicolai J Wewer Albrechtsen
发表日期
2020/12/1
期刊
Molecular metabolism
卷号
42
页码范围
101080
出版商
Elsevier
简介
Objective
Glucagon is well known to regulate blood glucose but may be equally important for amino acid metabolism. Plasma levels of amino acids are regulated by glucagon-dependent mechanism(s), while amino acids stimulate glucagon secretion from alpha cells, completing the recently described liver-alpha cell axis. The mechanisms underlying the cycle and the possible impact of hepatic steatosis are unclear.
Methods
We assessed amino acid clearance in vivo in mice treated with a glucagon receptor antagonist (GRA), transgenic mice with 95% reduction in alpha cells, and mice with hepatic steatosis. In addition, we evaluated urea formation in primary hepatocytes from ob/ob mice and humans, and we studied acute metabolic effects of glucagon in perfused rat livers. We also performed RNA sequencing on livers from glucagon receptor knock-out mice and mice with hepatic steatosis. Finally, we measured …
学术搜索中的文章
M Winther-Sørensen, KD Galsgaard, A Santos… - Molecular metabolism, 2020