作者
María V Girart, Mercedes B Fuertes, Carolina I Domaica, Lucas E Rossi, Norberto W Zwirner
发表日期
2007/9/15
期刊
The Journal of Immunology
卷号
179
期号
6
页码范围
3472-3479
出版商
American Association of Immunologists
简介
NK cells express different TLRs, such as TLR3, TLR7, and TLR9, but little is known about their role in NK cell stimulation. In this study, we used specific agonists (poly (I: C), loxoribine, and synthetic oligonucleotides containing unmethylated CpG sequences to stimulate human NK cells without or with suboptimal doses of IL-12, IL-15, or IFN-α, and investigated the secretion of IFN-γ, cytotoxicity, and expression of the activating receptor NKG2D. Poly (I: C) and loxoribine, in conjunction with IL-12, but not IL-15, triggered secretion of IFN-γ. Inhibition of IFN-γ secretion by chloroquine suggested that internalization of the TLR agonists was necessary. Also, secretion of IFN-γ was dependent on MEK1/ERK, p38 MAPK, p70 S6 kinase, and NF-κB, but not on calcineurin. IFN-α induced a similar effect, but promoted lesser IFN-γ secretion. However, cytotoxicity (51 Cr release assays) against MHC class I-chain related A (MICA …
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