作者
Aleksandra Majchrzak-Celińska, Małgorzata Zielińska-Przyjemska, Marcin Wierzchowski, Robert Kleszcz, Elżbieta Studzińska-Sroka, Mariusz Kaczmarek, Jarosław Paluszczak, Judyta Cielecka-Piontek, Violetta Krajka-Kuźniak
发表日期
2021/3/1
期刊
Advances in Medical Sciences
卷号
66
期号
1
页码范围
6-20
出版商
Elsevier
简介
Purpose
Glioblastoma is the most common and the deadliest brain cancer. The aim of this study was to analyze the impact of resveratrol and its five analogs: 3,4,4′-trimethoxy, 3,4,2′-trimethoxy, 3,4,2′,4′-tetramethoxy, 3,4,2′,6′-tetramethoxy and 3,4,2′,4′,6′-pentamethoxy-trans-stilbenes (MS) on human glioblastoma T98G cells.
Materials and methods
The Parallel Artificial Membrane Permeation Assay (PAMPA) was used for the prediction of blood-brain barrier penetration ability of the tested stilbenes (PAMPA-BBB). MTT test was applied to analyze the cytotoxicity of the compounds, whereas their ability to inhibit Wnt/β-catenin target genes expression was verified using qPCR. The potential DNA demethylating properties of the analyzed compounds were tested by Methylation-Sensitive High Resolution Melting (MS-HRM). Cell cycle distribution was tested using Fluorescence-Activated Cell Sorting (FACS …
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A Majchrzak-Celińska, M Zielińska-Przyjemska… - Advances in Medical Sciences, 2021