作者
Gabriela Trevisan, Carin Hoffmeister, Mateus F Rossato, Sara M Oliveira, Mariane A Silva, Rafael P Ineu, Gustavo P Guerra, Serena Materazzi, Camilla Fusi, Romina Nassini, Pierangelo Geppetti, Juliano Ferreira
发表日期
2013/11
期刊
Arthritis & Rheumatism
卷号
65
期号
11
页码范围
2984-2995
简介
Objective
Gout is a common cause of inflammatory arthritis and is provoked by the accumulation of monosodium urate (MSU) crystals. However, the underlying mechanisms of the pain associated with acute attacks of gout are poorly understood. The aim of this study was to evaluate the role of transient receptor potential ankyrin 1 (TRPA‐1) and TRPA‐1 stimulants, such as H2O2, in a rodent model of MSU‐induced inflammation.
Methods
MSU or H2O2 was injected into the hind paws of rodents or applied in cultured sensory neurons, and the intracellular calcium response was measured in vitro. Inflammatory or nociceptive responses in vivo were evaluated using pharmacologic, genetic, or biochemical tools and methods.
Results
TRPA‐1 antagonism, TRPA‐1 gene deletion, or pretreatment of peptidergic TRP‐expressing primary sensory neurons with capsaicin markedly decreased MSU‐induced nociception and …
学术搜索中的文章
G Trevisan, C Hoffmeister, MF Rossato, SM Oliveira… - Arthritis & Rheumatism, 2013