作者
Olle Terenius, Alexie Papanicolaou, Jennie S Garbutt, Ioannis Eleftherianos, Hanneke Huvenne, Sriramana Kanginakudru, Merete Albrechtsen, Chunju An, Jean-Luc Aymeric, Andrea Barthel, Piotr Bebas, Kavita Bitra, Alejandra Bravo, François Chevalier, Derek P Collinge, Cristina M Crava, Ruud A de Maagd, Bernard Duvic, Martin Erlandson, Ingrid Faye, Gabriella Felföldi, Haruhiko Fujiwara, Ryo Futahashi, Archana S Gandhe, Heather S Gatehouse, Laurence N Gatehouse, Jadwiga M Giebultowicz, Isabel Gómez, Cornelis JP Grimmelikhuijzen, Astrid T Groot, Frank Hauser, David G Heckel, Dwayne D Hegedus, Steven Hrycaj, Lihua Huang, J Joe Hull, Kostas Iatrou, Masatoshi Iga, Michael R Kanost, Joanna Kotwica, Changyou Li, Jianghong Li, Jisheng Liu, Magnus Lundmark, Shogo Matsumoto, Martina Meyering-Vos, Peter J Millichap, Antónia Monteiro, Nirotpal Mrinal, Teruyuki Niimi, Daniela Nowara, Atsushi Ohnishi, Vicencio Oostra, Katsuhisa Ozaki, Maria Papakonstantinou, Aleksandar Popadic, Manchikatla V Rajam, Suzanne Saenko, Robert M Simpson, Mario Soberón, Michael R Strand, Shuichiro Tomita, Umut Toprak, Ping Wang, Choon Wei Wee, Steven Whyard, Wenqing Zhang, Javaregowda Nagaraju, Salvador Herrero, Karl Gordon, Luc Swevers, Guy Smagghe
发表日期
2011/2/1
来源
Journal of insect physiology
卷号
57
期号
2
页码范围
231-245
出版商
Pergamon
简介
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi …
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O Terenius, A Papanicolaou, JS Garbutt, I Eleftherianos… - Journal of insect physiology, 2011
