作者
S Edwards, C Campbell, P Flohr, J Shipley, I Giddings, R Te-Poele, A Dodson, C Foster, J Clark, S Jhavar, G Kovacs, CS Cooper
发表日期
2005/1
期刊
British journal of cancer
卷号
92
期号
2
页码范围
376-381
出版商
Nature Publishing Group
简介
In a strategy aimed at identifying novel markers of human prostate cancer, we performed expression analysis using microarrays of clones randomly selected from a cDNA library prepared from the LNCaP prostate cancer cell line. Comparisons of expression profiles in primary human prostate cancer, adjacent normal prostate tissue, and a selection of other (nonprostate) normal human tissues, led to the identification of a set of clones that were judged as the best candidate markers of normal and/or malignant prostate tissue. DNA sequencing of the selected clones revealed that they included 10 genes that had previously been established as prostate markers: NKX3. 1, KLK2, KLK3 (PSA), FOLH1 (PSMA), STEAP2, PSGR, PRAC, RDH11, Prostein and FASN. Following analysis of the expression patterns of all selected and sequenced genes through interrogation of SAGE databases, a further three genes from our clone …
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S Edwards, C Campbell, P Flohr, J Shipley, I Giddings… - British journal of cancer, 2005