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The aim of this study was to evaluate the influence of the CO 2-specific flow rate on pH control, CO 2 use efficiency and biomass and lipid productivity of Nannochloropsis gaditana cultivated in outdoor tubular photobioreactors of 3.0 m 3. In that study was evaluated the injection of CO 2-specific flow rates ranging from 0.4 to 7.7 mL CO 2 L culture− 1 min− 1. The use of low CO 2-specific flow rates, from 0.4 to 1.9 mL CO 2 L culture− 1 min− 1, has enabled pH control. The highest biomass productivities and CO 2 use efficiency were obtained using the flow rates of 1.2 and 1.9 mL CO 2 L culture− 1 min− 1. The highest lipid productivities were verified in experiments with flow rates of 0.4 mL CO 2 L culture− 1 min− 1, followed by 1.9 mL CO 2 L culture− 1 min− 1. A high CO 2-specific flow rate increases CO 2 loss and reduces the performance of the cells due to inadequate pH control. By contrast, a low CO 2-specific flow …