作者
Nadina Nieves, Jose C Lorenzo, Maria de los A Blanco, Justo González, Hipólito Peralta, Martha Hernández, Ramón Santos, Oscar Concepción, Carlos G Borroto, Eduviges Borroto, Raúl Tapia, Marcos E Martinez, Zaida Fundora, Alfredo González
发表日期
1998/8
期刊
Plant Cell, Tissue and Organ Culture
卷号
54
期号
2
页码范围
77-83
出版商
Kluwer Academic Publishers
简介
Synthetic seed technology may be of value in breeding programs and allow the propagation of many elite genotype-derived plants in a short time. In this work, a range of artificial endosperm treatments of Cleopatra tangerine zygotic embryos were evaluated for suitability for encapsulation of somatic embryos. Different complexing ions in the form of alginate capsules, zeolite as an ion exchanger and the relationship between capsule-nutrient gel on germination of zygotic embryos, were evaluated. Artificial endosperm assays showed that abscisic acid (1 μM) and mannitol (0.25 M) delayed germination and conversion of zygotic embryos, whereas amino acid supplements (proline, glutamic acid and arginine) accelerated the conversion process. An artificial endosperm was used to encapsulate somatic and zygotic embryos. After encapsulation, zygotic embryos germinated after four days of culture while somatic …
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