作者
Thomas S Elliott, Ambra Bianco, Fiona M Townsley, Stephen D Fried, Jason W Chin
发表日期
2016/7/21
期刊
Cell chemical biology
卷号
23
期号
7
页码范围
805-815
出版商
Elsevier
简介
Cell-specific proteomics in multicellular systems and whole animals is a promising approach to understand the differentiated functions of cells and tissues. Here, we extend our stochastic orthogonal recoding of translation (SORT) approach for the co-translational tagging of proteomes with a cyclopropene-containing amino acid in response to diverse codons in genetically targeted cells, and create a tetrazine-biotin probe containing a cleavable linker that offers a way to enrich and identify tagged proteins. We demonstrate that SORT with enrichment, SORT-E, efficiently recovers and enriches SORT tagged proteins and enables specific identification of enriched proteins via mass spectrometry, including low-abundance proteins. We show that tagging at distinct codons enriches overlapping, but distinct sets of proteins, suggesting that tagging at more than one codon enhances proteome coverage. Using SORT-E, we …
学术搜索中的文章
TS Elliott, A Bianco, FM Townsley, SD Fried, JW Chin - Cell chemical biology, 2016