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MATERIALS AND METHODS

Cells, plasmids, and antibodies. 293T cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 2 mM

L-glutamine, penicillin (100 U/ml), and streptomycin (100 g/ml). The HTLV-1 proviral clone ACH (25) and HTLV-2 proviral clone, pH6neo (5), were used in this study. pME-p30-HA (a kind gift from B. Michael, Ohio State University) was generated from ORF II of the ACH proviral clone, tagged with hemagglutinin (HA) at the C terminus, and cloned into the expression vector pME-18S at the EcoRI and NotI sites. The protein was detected by Western blotting with anti-HA monoclonal antibody (Covance). Tax and Rex were expressed from a vector encoding the respective cDNA under the control of the cytomegalovirus immediate-early gene promoter that has been described previously (45). An HTLV-2 p28II expression vector (p28-AU1) was …