作者
Ihab Younis, Lyne Khair, Miroslav Dundr, Michael D Lairmore, Genoveffa Franchini, Patrick L Green
发表日期
2004/10/15
期刊
Journal of virology
卷号
78
期号
20
页码范围
11077-11083
出版商
American Society for Microbiology
简介
MATERIALS AND METHODS
Cells, plasmids, and antibodies. 293T cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 2 mM
L-glutamine, penicillin (100 U/ml), and streptomycin (100 g/ml). The HTLV-1 proviral clone ACH (25) and HTLV-2 proviral clone, pH6neo (5), were used in this study. pME-p30-HA (a kind gift from B. Michael, Ohio State University) was generated from ORF II of the ACH proviral clone, tagged with hemagglutinin (HA) at the C terminus, and cloned into the expression vector pME-18S at the EcoRI and NotI sites. The protein was detected by Western blotting with anti-HA monoclonal antibody (Covance). Tax and Rex were expressed from a vector encoding the respective cDNA under the control of the cytomegalovirus immediate-early gene promoter that has been described previously (45). An HTLV-2 p28II expression vector (p28-AU1) was …
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