作者
Aibin Zhan, Martin Hulák, Francisco Sylvester, Xiaoting Huang, Abisola A Adebayo, Cathryn L Abbott, Sarah J Adamowicz, Daniel D Heath, Melania E Cristescu, Hugh J MacIsaac
发表日期
2013/6
期刊
Methods in Ecology and Evolution
卷号
4
期号
6
页码范围
558-565
简介
- Concerns regarding the rapid loss of endemic biodiversity, and introduction and spread of non‐indigenous species, have focused attention on the need and ability to detect species present in communities at low abundance. However, detection of rare species poses immense technical challenges, especially for morphologically cryptic species, microscopic taxa and those beneath the water surface in aquatic ecosystems.
- Next‐generation sequencing technology provides a robust tool to assess biodiversity, especially for detection of rare species. Here, we assess the sensitivity of 454 pyrosequencing for detection of rare species using known indicator species spiked into existing complex plankton samples. In addition, we develop universal small subunit ribosomal DNA primers for amplification of a wide range of taxa for detailed description of biodiversity in complex communities.
- A universality test of newly …
学术搜索中的文章
A Zhan, M Hulák, F Sylvester, X Huang, AA Adebayo… - Methods in Ecology and Evolution, 2013