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Nickel and Copper nanoparticles were synthesized by simple chemical method and studied for antimicrobial activities. The size of synthesized Nickel and Copper nanoparticles was found to be 24.00 nm and 13.13 nm respectively. The XRD analysis reveals the crystal system of Nickel and Copper nanoparticles. Copper nanoparticles were found orthorhombic whereas the nickel nanoparticles were monoclinic. The antimicrobial activities of Nickel and Copper nanoparticles dispersed in DMSO was investigated. Comparative sensitivity test of these synthesized nanoparticles was carried out against three pathogenic micro-organisms (Gram negative bacteria), viz. Escherichia coli, Klebsiella pneumoniae and Pneumonia Typhus, using agar diffusion cup plate method. Copper and Nickel nanoparticles have shown appreciable sensitivity at 100 μg/ml against all test micro-organisms. Comparatively, Copper …