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This study evaluated the validity of the total energy expenditure (TEE) estimated using uniaxial (ACCuni) and triaxial (ACCtri) accelerometers in the elderly. Thirty-two healthy elderly (64–87 years) participated in this study. TEE was measured using the doubly labeled water (DLW) method (TEE_DLW). TEE_ACCuni (6.79 ± 1.08 MJ day⁻¹) was significantly lower than TEE_DLW (7.85 ± 1.54 MJ day⁻¹) and showed wider limits of agreement (−3.15 to 1.12 MJ day⁻¹) with a smaller correlation coefficient (r = 0.703). TEE_ACCtri (7.88 ± 1.27 MJ day⁻¹) did not differ from TEE_DLW and showed narrower limits of agreement (−1.64 to 1.72 MJ day⁻¹) with a larger correlation coefficient (r = 0.835, P < 0.001). The estimated intensities of light activities were significantly lower with ACCuni. Greater mediolateral acceleration was observed during 6-min walk tests. The results suggest that ACCtri is a better choice than ACCuni …