作者
Sara Aliakbari, Mobin Mohammadi, Mohammad Ali Rezaee, Abbas Ali Amini, Shohreh Fakhari, Mohammad Reza Rahmani
发表日期
2019
期刊
EXCLI journal
卷号
18
页码范围
852
出版商
Leibniz Research Centre for Working Environment and Human Factors
简介
The immunomodulatory properties of type 2 diabetic patients' adipose-derived mesenchymal stem cells (D-ASCs) has not been extensively studied. In this study, we compared the immunomodulatory properties of D-ASCs and non-diabetic subjects mesenchymal stem cells (ND-ASCs) in co-culture with mixed leukocyte reaction (MLR). ASCs were isolated from adipose tissue samples of type 2 diabetic and non-diabetic subjects (age: 40-55). D-ASCs and ND-ASCs were co-cultured with two-way MLR. Peripheral blood mononuclear cells (PBMCs) proliferation ratio, protein levels of IFN-γ and IL-10, mRNA expression of COX-2, TNF-α, TGF-β1 and IL-6 genes in MLR, D-ASCs and ND-ASCs co-cultures were assessed using XTT, ELISA and Real-time qRT-PCR, respectively. PBMCs proliferation on days 2 and 4 of D-ASCs co-culture was higher than ND-ASCs co-culture of the same days (p< 0.001). IFN-γ level …
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S Aliakbari, M Mohammadi, MA Rezaee, AA Amini… - EXCLI journal, 2019