作者
Hugo Pereira, Luísa Barreira, André Mozes, Cláudia Florindo, Cristina Polo, Catarina V Duarte, Luísa Custódio, João Varela
发表日期
2011/12
期刊
Biotechnology for biofuels
卷号
4
页码范围
1-12
出版商
BioMed Central
简介
We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.
学术搜索中的文章
H Pereira, L Barreira, A Mozes, C Florindo, C Polo… - Biotechnology for biofuels, 2011