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Soil pollution caused by heavy metals is one of the major problems throughout the world. To maintain a safe and healthy environment for human beings, there is a dire need to identify hyperaccumulator plants and the underlying genes involved in heavy metals stress tolerance and accumulation. The goal of this research is to explore the potential of hemp as a decontaminator of heavy metals by identifying the two important heavy metals responsive genes, glutathione‐disulfidereductase (GSR) and phospholipase D‐α (PLDα). The results revealed heavy metals accumulation; Cu (1530 mg kg⁻¹), Cd (151 mg kg⁻¹), and Ni (123 mg kg⁻¹) in hemp plants’ leaves collected from the contaminated site. This shows the ability of the hemp plant to tolerate heavy metals, perhaps due to the presence of stress tolerance genes. In this study, partial sequences of putative GSR (215 bp) and PLDα (517 bp) genes were …