作者
Meredith JH Hutton, Clara Westwell-Roper, Galina Soukhatcheva, Annette Plesner, Jan P Dutz, C Bruce Verchere
发表日期
2009/11/15
期刊
Transplantation
卷号
88
期号
9
页码范围
1075-1080
出版商
LWW
简介
Background.
Islet transplantation is a promising therapy for type 1 diabetes; however, most islet grafts fail within 5 years. Innate immunity has been suggested to play a role in islet allograft rejection, potentially mediated by toll-like receptors (TLRs), a class of innate immune receptors. Lack of TLR4, in particular, has been reported to improve allograft survival. Therefore, we hypothesized that TLRs may be involved in islet allograft rejection, and that deletion of TLR4 may improve islet graft survival.
Methods.
Islets were isolated from C57BL/10ScNJ (Tlr4−/−) and C57BL/10 (wild-type [WT]) animals and transplanted into Balb/cJ recipients with streptozotocin-induced diabetes. Blood glucose levels were used to determine graft viability and immunostaining to assess graft morphology and immune cell infiltration. The roles of the TLR4 adaptor molecules MyD88 and TLR adaptor molecule 1 (Ticam-1) were assessed using …
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MJH Hutton, C Westwell-Roper, G Soukhatcheva… - Transplantation, 2009