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The present study was conducted to study the production and evaluation of potentially probiotic additives containing both live lactic acid bacteria (Pediococcus acidilactici NRRL B-5627, Lactococcus lactis subsp. lactis CECT 539, Lactobacillus casei subsp. casei CECT 4043 and Enterococcus faecium CECT 410) and antimicrobial metabolites with could be used as a replacement for antibiotics in weanling pig diets. The gastrointestinal transit tolerance of the four bacteria was determined by exposing washed cell suspensions at 30°C to acidic conditions (pH 1.0, 2.0, 3.0, 4.0 and 5.0), to a simulated gastric juice (pH 2.0) containing pepsin (3g/L) and sodium chloride (5g/L), and to a simulated small intestinal juice (pH 8.0) containing pancreatin (1g/L) and sodium chloride (5g/L), mimicking the gastrointestinal environment. These studies showed that the four strains are capable of surviving the passage through the …