作者
Arya Biragyn, Igor M Belyakov, Yen-Hung Chow, Dimiter S Dimitrov, Jay A Berzofsky, Larry W Kwak
发表日期
2002/8/15
期刊
Blood, The Journal of the American Society of Hematology
卷号
100
期号
4
页码范围
1153-1159
出版商
American Society of Hematology
简介
DNA immunizations with glycoprotein 120 (gp120) of human immunodeficiency virus–1 (HIV-1) usually require boosting with protein or viral vaccines to achieve optimal efficacy. Here, we demonstrate for the first time that mice immunized with DNA encoding gp120 fused with proinflammatory chemoattractants of immature dendritic cells, such as β-defensin 2, monocyte chemoattractant protein–3 (MCP-3/CCL7) or macrophage-derived chemokine (MDC/CCL22), elicited anti-gp120 antibodies with high titers of virus-neutralizing activity. The immunogenicity was further augmented with the use of chemokine fusion constructs with gp140, gp120 linked to the extracellular domain of gp41 via a 14–amino acid spacer peptide sequence. This construct elicited antibodies with more effective neutralizing activity than corresponding constructs expressing gp120. Responses were dependent on physical linkage with chemokine …
引用总数
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