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The relative acute toxicity and sub-lethal effects of two surfactants; Faab liquid soap (FLS) and Morning Fresh liquid soap (MFLS) were evaluated for the Poecilia reticulata in laboratory bioassays. The acute toxicity was evaluated for 96 h while the sub-lethal effects of the two surfactants on the biochemical parameters of P. reticulata were investigated for a 6–day exposure period. The derived toxicity indices showed that the FLS (96hLC50= 0.062 ml/L) was 1.37 times more toxic than the MFLS (96hLC50= 0.085 ml/L) against P. reticulata. Analysis of variance (ANOVA) showed significant difference (p< 0.05) in the quantal response of P. reticulata to different concentrations of FLS and MFLS at 24, 48, 72 and 96 h of exposure. The mean tissue protein, triglyceride, glucose and cholesterol ranged from (21.04-48.54 and 18.06-58.54 mg/dl),(87.43-154.95 and 85.63-144.28 mg/dl),(33.95-55.03 and 32.76-53.15 mg/dl) and (37.30-65.38 and 37.12-58.11 mg/dl) for FLS and MFLS respectively. ANOVA showed significant difference (p< 0.05) in the biochemical response of P. reticulata to FLS and MFLS. The implications of these findings are highlighted.