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Ultrasonic manipulation, which uses acoustic radiation forces, is a contactless manipulation technique. It allows the simultaneous handling of single or numerous particles (eg, copolymer beads, biological cells) suspended in a fluid, without the need for prior localization. Here it is reported on a method for two-dimensional arraying based on the superposition of two in-plane orthogonally oriented standing pressure waves. A device has been built and the experimental results have been compared with a qualitative analytical model. A single piezoelectric transducer is used to excite the structure to vibration, which consists of a square chamber etched in silicon sealed with a glass plate. A set of orthogonally aligned electrodes have been defined on one surface of the piezoelectric. This allows either a quasi-one-dimensional standing pressure field to be excited in one of two directions or if both electrodes are activated …