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(CMCase), Filter paperase (FPase) and βglucosidase (BGL)} are hydrolytic enzymes capable of degradation of cellulose for production of variety of value added products. In the present study a novel strain of fungus identified as Aspergillus niger RSO-1 was used to examine cellulase production by using wheat bran (WB), rice bran (RB) and saw dust (SD) as substrates in submerged (SmF), surface (SF) and solid state fermentations (SSF). Time course studies were performed and considerable amount of all three enzymes was observed after 4 days of incubation (CMCase, 2.9 IU/ml; FPase, 1.49 IU/ml; BGL, 3.29 IU/ml) using WB as substrate in SSF. Optimization was carried out with WB using SSF for physiochemical parameters including incubation temperature, inoculum size, moisture content, exogenous addition of carbon sources, nitrogen sources, surfactants and mineral sources.