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Grape pomace (pulp and skins) was investigated as a new biosorbent for removing mycotoxins from liquid media. In vitro adsorption experiments showed that the pomace obtained from Primitivo grapes is able to sequester rapidly and simultaneously different mycotoxins. Aflatoxin B₁ (AFB₁) was the most adsorbed mycotoxin followed by zearalenone (ZEA), ochratoxin A (OTA), and fumonisin B₁ (FB₁), whereas the adsorption of deoxynivalenol (DON) was negligible. AFB₁ and ZEA adsorptions were not affected by changing pH values in the pH 3–8 range, whereas OTA and FB₁ adsorptions were significantly affected by pH. Equilibrium adsorption isotherms obtained at different temperatures (5–70 °C) and pH values (3 and 7) were modeled and evaluated using the Freundlich, Langmuir, Sips, and Hill models. The goodness of the fits and the parameters involved in the adsorption mechanism were calculated by the …