作者
Eiko Miura, Yusuke Kato, Ryo Matsushima, Verónica Albrecht, Soumaya Laalami, Wataru Sakamoto
发表日期
2007/4/1
期刊
The Plant Cell
卷号
19
期号
4
页码范围
1313-1328
出版商
American Society of Plant Biologists
简介
An Arabidopsis thaliana leaf-variegated mutant yellow variegated2 (var2) results from loss of FtsH2, a major component of the chloroplast FtsH complex. FtsH is an ATP-dependent metalloprotease in thylakoid membranes and degrades several chloroplastic proteins. To understand the role of proteolysis by FtsH and mechanisms leading to leaf variegation, we characterized the second-site recessive mutation fu-gaeri1 (fug1) that suppressed leaf variegation of var2. Map-based cloning and subsequent characterization of the FUG1 locus demonstrated that it encodes a protein homologous to prokaryotic translation initiation factor 2 (cpIF2) located in chloroplasts. We show evidence that cpIF2 indeed functions in chloroplast protein synthesis in vivo. Suppression of leaf variegation by fug1 is observed not only in var2 but also in var1 (lacking FtsH5) and var1 var2. Thus, suppression of leaf variegation caused by …
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E Miura, Y Kato, R Matsushima, V Albrecht, S Laalami… - The Plant Cell, 2007