作者
Huaxin Chen, Jianye Cai, Jiancheng Wang, Yuan Qiu, Chenhao Jiang, Yi Wang, Yiqin Wang, Chenju Yi, Lijie Pan, Yuanjun Guan, Jun Zheng, Dongbo Qiu, Cong Du, Qiuli Liu, Guihua Chen, Yang Yang, Yan Xu, Andy Peng Xiang, Qi Zhang
发表日期
2021/5/1
期刊
Journal of hepatology
卷号
74
期号
5
页码范围
1176-1187
出版商
Elsevier
简介
Background & Aims
Liver fibrosis is a wound healing response that arises from various aetiologies. The intermediate filament protein Nestin has been reported to participate in maintaining tissue homeostasis during wound healing responses. However, little is known about the role Nestin plays in liver fibrosis. This study investigated the function and precise regulatory network of Nestin during liver fibrosis.
Methods
Nestin expression was assessed via immunostaining and quantitative real-time PCR (qPCR) in fibrotic/cirrhotic samples. The induction of Nestin expression by transforming growth factor beta (TGFβ)-Smad2/3 signalling was investigated through luciferase reporter assays. The functional role of Nestin in hepatic stellate cells (HSCs) was investigated by examining the pathway activity of profibrogenic TGFβ-Smad2/3 signalling and degradation of TGFβ receptor I (TβRI) after interfering with Nestin. The in vivo …
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