作者
Yuh-Tzy Lin, Jai-Sing Yang, Hui-Ju Lin, Tzu-Wei Tan, Nou-Ying Tang, Jo-Hua Chaing, Yung-Hsien Chang, Hsu-Feng Lu, Jing-Gung Chung
发表日期
2007/11/1
期刊
In vivo
卷号
21
期号
6
页码范围
1053-1058
出版商
International Institute of Anticancer Research
简介
Background
The effects of baicalein on SCC-4 human tongue cancer cells were examined to better understand its effect on apoptosis and associated possible signal pathways in vitro. Materials and Methods
Apoptosis induction, reactive oxygen species (ROS), cytoplasmic Ca2+, mitochondrial membrane potential (MMP) and caspase-3 activity were analyzed using the flow cytometric assay. Apoptosis-associated proteins, such as p53, BAX, BCL-2, cytochrome c, caspase-3 and -9, EndoG and AIF were determined by Western blotting. Results
Our results showed that baicalein promoted the levels of p53, BAX, cytochrome c, capase-3 and -9 and reduced the level of BCL-2, which were associated with the induction of apoptotic cell death of SCC-4 cells. A release of cytochrome c from mitochondria into cytosol was demonstrated and an activation of caspase-3, which led to the occurrence of apoptosis in SCC-4 cells …学术搜索中的文章
YT Lin, JS Yang, HJ Lin, TW Tan, NY Tang, JH Chaing… - In vivo, 2007