查看文章

Phosphorus uptake and translocation through intact mycelial systems of Paxillus involutus and Suillus variegatus infecting Pinus contorta seedlings was monitored non-destructively using a β-scanner. Mycorrhizal plants were grown in flat perspex chambers (20 × 6 cm²) and root growth was restricted to the upper portion of each chamber enabling mycelial translocation to be studied over distances of up to 15 cm. ³²P was supplied, either directly to distal parts of the extending mycelium, or to single, cut mycelial strands in feeding dishes. Two-dimensional patterns of activity were accumulated as scans with a lateral resolution of 5 mm and a longitudinal resolution of 3–4 mm. No distinct translocation front could be detected but patterns of accumulation of label in the mycorrhizal roots were not consistent with movement by simple diffusion. Activity in translocating hyphae became visible only after the activity in …