作者
JJ Vargas-Magaña, JFJ Torres-Acosta, AJ Aguilar-Caballero, CA Sandoval-Castro, H Hoste, JI Chan-Pérez
发表日期
2014/12/15
期刊
Veterinary Parasitology
卷号
206
期号
3-4
页码范围
322-327
出版商
Elsevier
简介
This study aimed at (i) describing the effects of acetone–water extracts obtained from a range of different plant materials, on the hatching process of Haemonchus contortus eggs under in vitro conditions and (ii) identifying the role of tannins and other plant secondary compounds (PSC), on these AH effects by using polyvinylpolypyrrolidone (PVPP), an inhibitor of tannins and other polyphenols. An egg hatch assay (EHA) was used to determine the AH effect. Acetone–water (70:30) extracts from different foliages (Lysiloma latisiliquum, Laguncularia racemosa, Rizophora mangle, Avicennia germinans) and plant by-products (Theobroma cacao seed husk and pulp, and percolated Coffea arabica) were obtained. Fresh H. contortus eggs were incubated in PBS with increasing concentrations of each extract (0, 600, 1200, 2400 and 3600 μg/ml PBS). A general linear model was used to determine the dose effect of each …
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