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This study was conducted to isolate and identify organisms responsible for sub-clinical mastitis (SCM) in goats and sheep for the determination of point prevalence of SCM and antibiotic sensitivity of the identified organisms. For this purpose 50 each of lactating sheep and goats were examined with the commercially available Leucocytest® SCM detection kit. It was found that 4 and 36% sheep and goats suffered from SCM, respective. The prevalence of clinical mastitis (CM) was 4 and 6% in sheep and goats, respectively. Milk samples were collected individually from sheep and goats with SCM and were cultured in different media including nutrient agar, blood agar and eosin methylene blue agar. The bacteria were further characterized by biochemical tests. In both goats and sheep, the organisms responsible for SCM were Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The organisms were …