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Biodiesel is still mainly produced from different vegetable (e.g., rapeseed, palm, soybean, sunflower, and used cooking oils) oils and animal fats, and therefore it has a negative impact on food and feed prices. For biodiesel production as an alternative feedstock, lipids from oleaginous microorganisms could be used. One of the oleaginous microorganisms is yeast Trichosporon oleaginosus that has the capacity to grow and accumulate lipids on different lignocellulosic hydrolysates. In this study, corn cobs were pretreated by alkali at loading from 0.08 to 1.6 g/g dry weight of untreated (raw) corn cobs (g NaOH/g_{DW_UCC}) at 121 °C for 30 min. In a further step, alkaline pretreated corn cobs were subjected to the enzymatic hydrolysis by using commercial multienzymes mixtures. The optimal alkali loading (0.16 g/g_{DW_UCC}) was related to the highest glucose and xylose yields which were observed during enzymatic …