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Organic nitrogen is omnipresent in nature. The variations of dissolved organic nitrogen (DON) concentration are usually a result of microbial activity, anthropogenic influence, and/or other physical-chemical processes (eg photo-mineralization). Therefore, DON serves as an important indicator for aquatic life cycles and water qualities. However, current DON detection methods inevitably produce exaggerated standard deviation, due to their inherent subtraction processes, as shown in the below equation. Consequently, they are incapable of propelling further understandings of the DON occurrence, DON role in nutrient recycling, and DON potential human toxicity as well. SDON=(STN2+ SDIN^ 2) 0.5, where: S= standard deviation in an effort to overcome the barricade, the primary goal of this study is to develop a membrane pretreatment unit using electro-dialysis (EDI) process. Similar to the well-known mechanism …