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Cover Photo:“Cell-type characterization of ECMf-mediated primary human prostate cell cultures.(A) Fresh primary prostate cells were seeded onto extracellular matrix-coated surfaces incubated overnight at 37 C with 5% CO2. The cells were stained with PSMA as an epithelial cell marker, cytokeratin 8+ 18 as a luminal epithelial cell marker, and alpha-smooth muscle actin as a fibroblast marker. Controls were treated with secondary antibody only and treated with the same conditions as the primary antibodies. All images are at 20× magnification.(B) Graph of percent of each type of cell found in the culture system. Five hundred cells were counted as either epithelial, luminal, or fibroblasts based on staining patterns for cultures derived from RP tissue samples (n= 3 samples). Then the percentage of each cell type was calculated. Graph shows the percentage of each cell type. DIC, differential interference contrast; ECMf …