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The present study evaluated polluted river water as a medium for the growth of oleaginous microalgae under mixotrophic conditions. Microalgae grow in the medium and produce biomass, pigments, and lipids with the removal of pollution loads from wastewater. Selenastrum sp. SL7 produced maximum biomass and lipids of 660 mg L^-1 and 194.5 mg L^-1, respectively. Fatty acid profiling data showed that elevated saturated fatty acid production and major fatty acids found in lipid from these algae were palmitic acids, oleic acid, stearic acid, linolenic acid, and linoleic acid. The low percentage of polyunsaturated fatty acids of EPA was also detected. Water quality in terms of pH, DO, TDS, COD, and BOD was significantly improved. The use of this medium for microalgae cultivation not only improves the biomass and lipid yields but also serves as an excellent means of phycoremediation of pollutants in waste streams …