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We present a preliminary list of the Culicidae species collected in seven localities at the province of Misiones, Argentina, since the mosquito fauna of this region is poorly known. Mosquito sampling was carried out during spring-summer, between August 2006 and January 2007, the months of highest abundance of these insects. Collection of specimens was made with a CDC-like trap (Center for Disease Control and Prevention) in peri-urban zones. Sixty-five species were identified. The geographic distribution of Anopheles darlingi, Uranotaenia davisi and Howardina fulvithorax is extended.