作者
Jan Naujoks, Christoph Tabeling, Brian D Dill, Christine Hoffmann, Andrew S Brown, Mareike Kunze, Stefan Kempa, Andrea Peter, Hans-Joachim Mollenkopf, Anca Dorhoi, Olivia Kershaw, Achim D Gruber, Leif E Sander, Martin Witzenrath, Susanne Herold, Andreas Nerlich, Andreas C Hocke, Ian van Driel, Norbert Suttorp, Sammy Bedoui, Hubert Hilbi, Matthias Trost, Bastian Opitz
发表日期
2016/2/1
期刊
PLoS pathogens
卷号
12
期号
2
页码范围
e1005408
出版商
Public Library of Science
简介
Macrophages can be niches for bacterial pathogens or antibacterial effector cells depending on the pathogen and signals from the immune system. Here we show that type I and II IFNs are master regulators of gene expression during Legionella pneumophila infection, and activators of an alveolar macrophage-intrinsic immune response that restricts bacterial growth during pneumonia. Quantitative mass spectrometry revealed that both IFNs substantially modify Legionella-containing vacuoles, and comparative analyses reveal distinct subsets of transcriptionally and spatially IFN-regulated proteins. Immune-responsive gene (IRG)1 is induced by IFNs in mitochondria that closely associate with Legionella-containing vacuoles, and mediates production of itaconic acid. This metabolite is bactericidal against intravacuolar L. pneumophila as well as extracellular multidrug-resistant Gram-positive and -negative bacteria. Our study explores the overall role IFNs play in inducing substantial remodeling of bacterial vacuoles and in stimulating production of IRG1-derived itaconic acid which targets intravacuolar pathogens. IRG1 or its product itaconic acid might be therapeutically targetable to fight intracellular and drug-resistant bacteria.
引用总数
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